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Detection of biomarkers for filoviral infection with a silicon photonic resonator platform. | LitMetric

AI Article Synopsis

  • The protocol outlines how to use silicon photonic microring resonator sensors to detect specific proteins of Ebola and Sudan viruses in blood samples.
  • It includes steps for preparing the sensors, detecting the biomarkers, creating calibration standards, and analyzing the results.
  • This method can also be modified to detect other substances like cytokines, chemokines, nucleic acids, and additional viruses.

Article Abstract

This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515683PMC
http://dx.doi.org/10.1016/j.xpro.2022.101719DOI Listing

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