AI Article Synopsis
- The plant cell wall is made up of different macromolecules that vary in amount and location, playing essential roles in the structure of plants.
- High-resolution live cell imaging is a key method for studying these components in plant biology effectively.
- A protocol is provided for preparing samples and capturing live images of cell wall components using readily available dyes, ensuring minimal stress to the plants during the process.
Article Abstract
The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.
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| http://dx.doi.org/10.1007/978-1-0716-2675-7_22 | DOI Listing |
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