Problem: The maternal-fetal immune abnormalities can result in recurrent spontaneous abortion (RSA). The role of NKG2C and LILRB1 pNK subsets in predicting pregnancy loss is uncertain.
Method Of Study: In this study, we aimed to compare the percentage of CD3 CD56 NK cells, NKG2C NK cells, and LILRB1 NK cells in peripheral blood between healthy pregnant women (HC group), RSA women followed by normal pregnancy (RSA-N group) and RSA women followed by abortion (RSA-A group) in the first trimester via flow cytometry, and explore the prediction value of NKG2C and LILRB1 NK cells for pregnancy loss in RSA via ROC curve. The MFI of NKG2C and LILRB1 of dNK were compared between and HC and RSA-A groups.
Results: The percentage of CD3-CD56 pNK cells between HC, RSA-N, and RSA-A groups shows no significant difference. In peripheral blood, the percentage of NKG2C NK cells were significantly increased in the RSA-A group than HC group and RSA-N group, and the percentage of LILRB1 NK cell were significantly decreased in the RSA-A group. The MFI of NKG2C and LILRB1 of dNK showed a similar trend with peripheral blood between HC and RSA-A groups. The NKG2C and LILRB1 NK cells were an independent risk factor for predicting pregnancy loss in RSA patients, with an area under the ROC curves (AUC) of .77 and .71 respectively.
Conclusion: Women with recurrent spontaneous abortion have abnormal NKG2C and LILRB1 pNK subsets, which could reflect immune abnormalities at the maternal-fetal interface, and NKG2C and LILRB1 pNK subsets could be a good indicator for the prediction of pregnancy loss.
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The predictive value of NKG2C+NK cells and LILRB1+NK cells in recurrent spontaneous abortion.
Am J Reprod Immunol
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Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.
Problem: The maternal-fetal immune abnormalities can result in recurrent spontaneous abortion (RSA). The role of NKG2C and LILRB1 pNK subsets in predicting pregnancy loss is uncertain.
Method Of Study: In this study, we aimed to compare the percentage of CD3 CD56 NK cells, NKG2C NK cells, and LILRB1 NK cells in peripheral blood between healthy pregnant women (HC group), RSA women followed by normal pregnancy (RSA-N group) and RSA women followed by abortion (RSA-A group) in the first trimester via flow cytometry, and explore the prediction value of NKG2C and LILRB1 NK cells for pregnancy loss in RSA via ROC curve.
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