AI Article Synopsis
- - The study focuses on Rv0233, a component of the ribonucleotide reductase, and its impact on the survival of a significant global pathogen under stress.
- - Researchers created a deletion strain of Rv0233 to assess its effects on growth and survival in various conditions, confirming that it is not vital for growth in oxygen-rich environments or when nitrite is present.
- - Results showed that removing Rv0233 did not change the strain's resistance to tuberculosis drugs or hydrogen peroxide, and it thrived equally well under nutrient scarcity and low oxygen, maintaining its ability to grow within macrophages.
Article Abstract
is an important global pathogen. We were interested in understanding the role of Rv0233, a proposed subunit of the class IB ribonucleotide reductase, and its role in surviving stress conditions. We constructed an in-frame, unmarked deletion strain of and characterized its growth and survival under replicating or non-replicating conditions. We confirmed previous studies that found that Rv0233 is not essential for aerobic growth or survival in the presence of nitrite. We demonstrated that the deletion of Rv0233 does not affect susceptibility to frontline tuberculosis drugs or hydrogen peroxide. The deletion strain survived equally well under nutrient starvation or in hypoxia and was not attenuated for growth in macrophages.
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Rv0233 is not essential for the survival of in stress conditions.
Microbiology (Reading)
September 2022
Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Article Synopsis
- - The study focuses on Rv0233, a component of the ribonucleotide reductase, and its impact on the survival of a significant global pathogen under stress.
- - Researchers created a deletion strain of Rv0233 to assess its effects on growth and survival in various conditions, confirming that it is not vital for growth in oxygen-rich environments or when nitrite is present.
- - Results showed that removing Rv0233 did not change the strain's resistance to tuberculosis drugs or hydrogen peroxide, and it thrived equally well under nutrient scarcity and low oxygen, maintaining its ability to grow within macrophages.
Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria.
J Bacteriol
February 2009
MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence for Biomedical TB Research, School of Pathology, University of the Witwatersrand, National Health Laboratory Service, Johannesburg 2000, South Africa.
Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pathogen also contains the nrdF1 (Rv1981c) and nrdB (Rv0233) genes, encoding an alternate class Ib RNR small (R2) subunit and a putative class Ic RNR R2 subunit, respectively.
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