AI Article Synopsis
- Research identifies several cross-protective antibodies that target a wide variety of influenza A virus strains and help eliminate the virus through immune mechanisms like Fcγ receptor (FcγR) activity and neutralizing effects.
- An original assay was developed to study how purified FI6 IgG interacts with cells presenting hemagglutinin (HA) using flow cytometry, with tests conducted on peripheral blood mononuclear cells from cynomolgus macaques.
- The study showed that after administering an influenza vaccine, classical monocytes, a specific type of immune cell, predominantly engaged with HA-expressing cells through the FcγR pathway, suggesting this assay could aid in creating a universal influenza
Article Abstract
Several cross-protective antibodies that recognize a broad range of influenza A virus (IAV) strains are known to have functions in virus elimination such as Fcγ receptor (FcγR)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the FcγR-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate purified FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by flow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the FcγR-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an FcγR-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal influenza vaccine.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486051 | PMC |
| http://dx.doi.org/10.1016/j.isci.2022.105085 | DOI Listing |
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