AI Article Synopsis
- PRRSV primarily infects alveolar macrophages and African green monkey kidney cells but struggles with certain strains like NADC30 and NADC34 in MARC-145 cells.
- The study highlights the role of scavenger receptor CD163, which enables various non-permissive cell lines to be infected by PRRSV.
- The findings suggest that porcine CD163-expressing macrophages effectively support infections from multiple PRRSV2 lineages, proving valuable for research and management of this disease, particularly given the current prevalence in China.
Article Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has a strict cell tropism. In addition to the primary alveolar macrophages, PRRSV is strictly cytotropic to African green monkey kidney cells, such as MARC-145 cells; however, MARC-145 cells are not infected by most NADC30-like and NADC34-like PRRSV strains. The essential scavenger receptor CD163 has been proved to mediate productive infection of PRRSV in various non-permissive cell lines. In this study, we systematically tested the porcine CD163 stably expressing 3D4/21 cells for infections with various PRRSV strains. The results showed that the porcine CD163-expressing macrophages support the infections of PRRSV2 of lineages 1, 5, and 8, as evidenced by Western blotting, immunofluorescence assay, quantitative PCR, and virus titration assay. Considering the current prevalence of NADC30-like and NADC34-like PRRSV2 of lineage 1 in China, the CD163-expressing macrophages are very useful for PRRSV research and disease management.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505768 | PMC |
| http://dx.doi.org/10.3390/v14092056 | DOI Listing |
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