Development of Novel Recombinant Antigens of Nucleoprotein and Matrix Proteins of Antigenicity and Structural Prediction.

Viruses

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Km 15.5 Carretera México-Toluca, Palo Alto, Cuajimalpa, Ciudad de México 05110, Mexico.

Published: September 2022

Blue eye disease (BED) is a swine viral infection that affects the pork industry of Mexico. (PRV) is the etiological agent, and the hemagglutinin-neuraminidase protein (HN) is characterized as the best antigen for serological tests, although other structural proteins, including the nucleoprotein (NP) and the matrix (M) protein, have been investigated during the infection of members of the family, generating promising results. Herein, for the first time, we successfully produced and characterized both the NP and M proteins of PRV by using a recombinant strategy in the heterologous system. The ORF of the NP and M genes were cloned in-frame with the pET-SUMO expression vector. Recombinant proteins proved to be a sensitive target to detect seroconversion at 7 days until 28 days in vaccinated mice (BALB/c) by indirect ELISAs. Immunoreactivity was also tested using porcine serum samples, in which antibodies were recognized from early stages to a persistence of PRV infection, which is indicative that these proteins contain properties similar to native antigens. The predicted tertiary structure showed that both proteins have a conserved structure that resembles those found in others . Our results pave the way for developing biotechnological tools based on these proteins for the control and prevention of BED.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504402PMC
http://dx.doi.org/10.3390/v14091946DOI Listing

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