Antioxidant, Antibacterial, and Antifungal Activities of the Ethanolic Extract Obtained from Roots and Leaves.

This work assessed the phenolic and flavonoid components and their antioxidant, antifungal, and antibacterial effects in the ethanolic extract of barberry leaf and roots. The antibactericidal activity of root and leaf extracts against pathogenic bacteria was tested using agar diffusion and microdilution broth production for the lowest inhibitory concentration (MIC). root and leaf extracts inhibited ATCC9973, HB101, , and Cip812. The disc assay technique was used to assess the bactericidal activity of the extracts versus both pathogenic Gram-positive and Gram-negative strains. Hydro alcoholic extract was more effective against bacterial than fungal strains. The results showed that leaf and roots extract had similar antifungal activities. root extract inhibited the mycelial growth of , , and . root extract has excellent antioxidant, antibacterial, and antifungal effects. exhibited antimicrobial activity in vitro, and MIC showed that parts efficiently affected pathogens in vitro. In conclusion, both roots and leaves have considerable antibacterial activity and can be used as a source of antibacterial, antioxidant, and bioactive compounds to benefit human health.