Maize is an important cereal crop which is severely affected by . The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains-namely, RAF5 and LAF5-were selected and identified as sp. MZ895490 and MZ895491, respectively. The bioprotective potential of was evaluated through bioassays. In a no-choice bioassay, second instar larvae of fed on treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg day) and consumptive (7.16 ± 3.48 mg mg day) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of was found to be greater due to inoculation with . than sp. The phenolics content of inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g). However, plant biomass production was more in sp inoculated treatment. The study thus demonstrates the potential utility of sp. and for improving growth and biotic () stress tolerance in maize.
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http://dx.doi.org/10.3390/microorganisms10091850 | DOI Listing |
ACS Nano
January 2025
Department of Botany and Plant Sciences, University of California, Riverside, California 92521, United States.
Nitrogen fertilizer delivery inefficiencies limit crop productivity and contribute to environmental pollution. Herein, we developed Zn- and Fe-doped hydroxyapatite nanomaterials (ZnHAU, FeHAU) loaded with urea (∼26% N) through hydrogen bonding and metal-ligand interactions. The nanomaterials attach to the leaf epidermal cuticle and localize in the apoplast of leaf epidermal cells, triggering a slow N release at acidic conditions (pH 5.
View Article and Find Full Text PDFBiotechnol Rep (Amst)
March 2025
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
The production of cannabinoid compounds such as Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabichromene (CBC) with potential pharmaceutical applications is growing sharply. However, challenges such as the low yield of minor cannabinoids, legal restrictions on cultivation, and the complexity and cost of purification from the Cannabis sativa plant necessitate a biotechnological approach. Since the biosynthetic pathway is disclosed, cannabinoids have been produced in yeast, insect cells and plants mainly by the heterologous expression of tetrahydrocannabinol acid synthase (THCAS).
View Article and Find Full Text PDFJ Extracell Vesicles
December 2024
Unit for Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Aachen, Germany.
Sci Rep
December 2024
Centre for Nanomaterials and Biotechnologies, Faculty of Science, Jan Evangelista Purkyně University in Ústí Nad Labem, Ústí Nad Labem, Czech Republic.
In recent years, there has been a growing interest in plant extracellular vesicles (pEVs) due to their immense potential for medical applications, particularly as carriers for drug delivery. To use the benefits of pEVs in the future, it is necessary to identify methods that facilitate their production in sufficient quantities while maintaining high quality. In this study, a comparative analysis of yields of tobacco pEV derived from apoplastic fluid, sterile calli, and suspension cultures, was performed to identify the most suitable plant material for vesicle isolation.
View Article and Find Full Text PDFPlant Physiol
December 2024
Department of Plant Physiology, Institute of Biology, Humboldt-Universität zu Berlin, Philippstr. 13 Building 12, 10115 Berlin, Germany.
The main phloem loader in potato, sucrose transporter StSUT1, is coexpressed with 2 members of the SWEET gene family: StSWEET11b, a clade III member of SWEET carriers assumed to be involved in sucrose efflux, and StSWEET1g, a clade I member involved in glucose efflux into the apoplast, that physically interacts with StSUT1. We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants.
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