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Miramides A-D: Identification of Detoxin-like Depsipeptides after Heterologous Expression of a Hybrid NRPS-PKS Gene Cluster from Lu17588. | LitMetric

AI Article Synopsis

  • Natural products from plants, fungi, and bacteria are valuable in medicine, agriculture, and food due to their diverse beneficial properties like antibacterial and anticancer effects.
  • Natural compounds have higher chemical diversity compared to synthetic compounds, making them important for developing new pharmaceuticals and prompting ongoing research.
  • The study reported the assembly and heterologous expression of a hybrid NRPS-PKS gene cluster from Lu17588 in Del14, leading to the production of four new compounds, miramides A-D, similar to known depsipeptides.

Article Abstract

Natural products derived from plants, fungi or bacteria have been used for years in the medicine, agriculture and food industries as they exhibit a variety of beneficial properties, such as antibiotic, antifungal, anticancer, herbicidal and immunosuppressive activities. Compared to synthetic compounds, natural products possess a greater chemical diversity, which is a reason why they are profitable templates for developing pharmaceutical drug candidates and ongoing research on them is inevitable. Performing heterologous expression with unknown gene clusters is the preferred method to activate gene clusters that are not expressed in the wild-type strain under laboratory conditions; thus, this method offers a way to discover new interesting metabolites. Here, we report the gene cluster assembly of a hybrid NRPS-PKS gene cluster from Lu17588, which was heterologously expressed in Del14. Four new compounds were produced by the obtained strain, which were named miramides A-D. Isolation and structure elucidation revealed similarity of the isolated compounds to the known depsipeptides rimosamides/detoxins.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503745PMC
http://dx.doi.org/10.3390/microorganisms10091752DOI Listing

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