Evaluation of Cytotoxic and Antibacterial Effect of Methanolic Extract of .

Background And Objectives: Bacterial antibiotics have had several side effects. Therefore, interest in natural substances with less side effects is increasing these days. , the root of , is used as a raw material for medicines. In this study, we investigated the antibacterial effect and the cytotoxicity of extract.

Materials And Methods: For cytotoxicity, MTT analysis according to ISO 10993-5 was performed. The antibacterial test of the was determined from bacterial viability, Inhibition zone test, CFU (colony forming unit) and SEM (scanning electron microscope). To confirm the antibacterial component of , the content of flavonoids and polyphenols was analyzed.

Results: Our results showed that extract contained flavonoids and polyphenols, which exhibited antimicrobial activity against () and (). Further, the cytotoxicity of extract was low.

Conclusions: We believe that our study makes a significant contribution to the literature because it demonstrates that extract can be used as an antibiotic.