Tea is an important economic crop and health beverage in China. The presence of heavy metal ions in tea poses a significant threat to public health. Here, we prepared an aptamer biosensor labelled with AIEgen nanospheres to detect Pb in tea. The dsDNA modified by amino and phosphoric acid was combined with the carboxylated AIEgen NPs to form AIEgen-DNA with a fluorescence group, which was then fixed to the surface of Zr-MOFs to quench the fluorescence of AIEgen NPs. At the same time, PEG was added to remove nonspecific adsorption. Then Pb was added to cut the DNA sequences containing the cutting sites, and AIEgen NPs and part of the DNA sequences were separated from the Zr-MOFs surface to recover the fluorescence. By comparing the fluorescence changes before and after adding Pb, the detection limit of Pb can reach 1.70 nM. The fluorescence sensor was applied to detect Pb in tea, and the detection results showed that the tea purchased on the market did not contain the concentration of Pb within the detection range. This study provides new insights into monitoring food and agriculture-related pollutants based on fluorescent biosensors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496512 | PMC |
| http://dx.doi.org/10.3390/bios12090745 | DOI Listing |
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