The Detection of Potential Native Probiotics spp. against Enteritidis, Infantis and Kentucky ST198 of Lebanese Chicken Origin.

continues to be a major threat to public health, especially with respect to strains from a poultry origin. In recent years, an increasing trend of antimicrobial resistance (AMR) in spp. was observed due to the misuse of antibiotics. Among the approaches advised for overcoming AMR, probiotics from the genus have increasingly been considered for use as effective prophylactic and therapeutic agents belonging to the indigenous microbiota. In this study, we isolated lactobacilli from the ilea and ceca of hens and broilers in order to evaluate their potential probiotic properties. Four species were identified as ( = 22, 45.8%), ( = 20, 41.6%), ( = 2, 4.2%) and ( = 1, 2%), while three other isolates ( = 3, 6.25%) were non-typable. Eight isolates, including ( = 4), ( = 2), ( = 1) and spp. ( = 1) were chosen on the basis of their cell surface hydrophobicity and auto/co-aggregation ability for further adhesion assays using the adenocarcinoma cell line Caco-2. The adhesion rate of these strains varied from 0.53 to 10.78%. A30/i26 and 16/c6 and 1/c24 showed the highest adhesion capacity, and were assessed for their ability to compete in and exclude the adhesion of to the Caco-2 cells. Interestingly, 16/c6 was shown to significantly exclude the adhesion of the three serotypes, . Enteritidis, . Infantis and . Kentucky ST 198, to Caco-2 cells. The results of the liquid co-culture assays revealed a complete inhibition of the growth of after 24 h. Consequently, the indigenous 16/c6 strain shows promising potential for use as a preventive probiotic added directly to the diet for the control of the colonization of spp. in poultry.