AI Article Synopsis
- Nitrate is a crucial nutrient and signaling molecule that helps regulate plant growth and metabolism.
- Researchers identified that the NIN-like protein (NLP) transcription factors are essential for plants' response to nitrate, with specific mutation in these proteins disrupting their growth and metabolic processes.
- The study revealed that NLP7 acts as a nitrate sensor, functioning similarly to a bacterial sensor, and demonstrated the significance of its structure in mediating plant responses to nitrate.
Article Abstract
Nitrate is an essential nutrient and signaling molecule for plant growth. Plants sense intracellular nitrate to adjust their metabolic and growth responses. Here we identify the primary nitrate sensor in plants. We found that mutation of all seven NIN-like protein (NLP) transcription factors abolished plants' primary nitrate responses and developmental programs. Analyses of NIN-NLP7 chimeras and nitrate binding revealed that NLP7 is derepressed upon nitrate perception via its amino terminus. A genetically encoded fluorescent split biosensor, mCitrine-NLP7, enabled visualization of single-cell nitrate dynamics in planta. The nitrate sensor domain of NLP7 resembles the bacterial nitrate sensor NreA. Substitutions of conserved residues in the ligand-binding pocket impaired the ability of nitrate-triggered NLP7 to control transcription, transport, metabolism, development, and biomass. We propose that NLP7 represents a nitrate sensor in land plants.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628810 | PMC |
| http://dx.doi.org/10.1126/science.add1104 | DOI Listing |
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