AI Article Synopsis
- An invasive pest poses a serious threat to horticultural vegetables due to its competitive edge and potential damage.
- Researchers cloned a chitin synthase (CHS) transcript belonging to the CHS2 family, finding its highest expression during the pupal stage with no significant differences among adult tissues.
- A new RNA interference method using dsRNA was developed, significantly reducing chitin synthase expression in pupae and resulting in a lower emergence rate, laying groundwork for future pest control strategies.
Article Abstract
is an important invasive pest that infects horticultural vegetables, displaying a strong competitive advantage and showing great potential for inflicting harm. Chitin synthase is one of the key enzymes in insect chitin metabolism and plays an important role in insect growth and development. In this study, a chitin synthase (CHS) transcript of was cloned, and the results showed that belongs to the CHS2 family. The expression analysis indicated the presence of the highest abundance of in the pupae at different developmental stages but showed no significant difference among different tissues in the adult. Furthermore, a dsRNA immersion method was developed for RNA interference (RNAi) in using transcript. RNAi can significantly reduce the expression of in pupae, and the emergence rate of the pupae was significantly lower than that of the control. The results provide a theoretical basis for exploring the role of chitin synthase gene in and proposing new pest control strategies.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504599 | PMC |
| http://dx.doi.org/10.3390/insects13090832 | DOI Listing |
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