Copper Chaperone for Superoxide Dismutase in May Be Associated with Feeding Adaptation after Host Shifting.

Western flower thrips () pose a serious threat to the global vegetable and flower crop production. The regulatory mechanism for superoxide dismutase (SOD) in the feeding adaptation of after host shifting remains unclear. In this study, the copper chaperone for SOD () and manganese SOD () genes in were cloned, and their expression levels at different developmental stages was determined. The mRNA expression of and in second-instar larvae and adult females of F, F, and F generations was analyzed after shifting the thrips to kidney bean and broad bean plants, respectively. The F and F second-instar larvae and F adult females showed significantly upregulated mRNA expression after shifting to kidney bean plants. The F second-instar larvae and F adult females showed significantly upregulated mRNA expression after shifting to broad bean plants. The RNA interference significantly downregulated the mRNA expression levels and adult females showed significantly inhibited SOD activity after shifting to kidney bean and broad bean plants. adult females subjected to RNA interference and released on kidney bean and broad bean leaves for rearing, respectively, significantly reduced the survival rate and fecundity. These findings suggest that plays an active role in regulating the feeding adaptation ability of after host shifting.