We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473185 | PMC |
| http://dx.doi.org/10.1039/c8na00331a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of early relapse in multiple myeloma patients.
Cell Div
January 2025
Babak Myeloma Group, Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Background: Multiple myeloma (MM) represents the second most common hematological malignancy characterized by the infiltration of the bone marrow by plasma cells that produce monoclonal immunoglobulin. While the quality and length of life of MM patients have significantly increased, MM remains a hard-to-treat disease; almost all patients relapse. As MM is highly heterogenous, patients relapse at different times.
View Article and Find Full Text PDFCirculating microRNAs and alcohol consumption in the Multiethnic Cohort Study.
Alcohol
January 2025
Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, CA; Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA; Center for Genetic Epidemiology, Keck School of Medicine, University of Southern California, Los Angeles, CA.
Excessive alcohol consumption is a significant public health concern and contributes to liver diseases and cancer. Modifiable lifestyle factors including alcohol consumption can influence circulating microRNAs (miRNAs), which are increasingly used as biomarkers for early disease detection. Yet limited studies have identified miRNAs associated with alcohol intake, particularly in multiethnic populations.
View Article and Find Full Text PDFDevelopment of novel nucleic acid therapy aimed at directly controlling liver fibrosis.
Mol Ther Nucleic Acids
March 2025
Department of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Currently, no drugs directly treat liver fibrosis. Previously, we have shown that treatment with miR-29a-3p improved liver fibrosis in a mouse model. To investigate the effectiveness of nucleic acid therapy at a lower dose, a modified nucleic acid was prepared based on miR-29a-3p.
View Article and Find Full Text PDFEMERALD in the making? A promising blood-based microRNA panel to detect oesophageal adenocarcinoma and Barrett's oesophagus.
Gut
January 2025
Gastroenterology and Hepatology, Mayo Clinic, Scottsdale, Arizona, USA
Herbal decoctions always contain numerous plant microRNAs (miRNAs), and some of these can be absorbed orally to exert cross-kingdom gene regulation. However, little is known about which specific types of herbal decoction-borne plant miRNAs are more likely to be absorbed. Thus, two antiviral herbal decoctions, Qingfei Paidu (QFPD) and Qingre Huashi Kangdu (QRHS), were administered to human volunteers and rats, respectively, to investigate the characteristics of orally absorbed decoction-borne plant miRNAs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!