We report the identification of the biosynthetic cluster from the marine-derived fungus and the in vivo characterization of a cryptic type I diterpene synthase. The heterologous expression of the bifunctional terpene synthase led to the discovery of a diterpene backbone, talarodiene, harboring a benzo[]cyclopenta[]cyclooctane tricyclic fused ring system. The conversion of geranylgeranyl diphosphate to talarodiene was investigated using C-labeling studies, and stable isotope tracer experiments showed the biotransformation of talarodiene into talaronoid C.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531244PMC
http://dx.doi.org/10.1021/acs.orglett.2c02904DOI Listing

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