Cannabidiol (CBD), the main nonpsychoactive cannabinoid in , has diverse applications in the pharmacological, food, and cosmetic industries. The long plantation period and the complex chemical structure of cannabidiol pose a great challenge on CBD supply. Here, we achieved biosynthesis of cannabidiol in . The CBD production was further enhanced by 2.53-fold through pushing the supply of precursors and fusion protein construction. Bile pigment transporter 1 (BPT1) was the most effective transporter for transferring cannabigerolic acid (CBGA) from the cytoplasm to the vacuole, which removed the physical barrier separating CBGA and its catalytic enzyme. The lowest binding energy of the CBGA-BPT1 complex confirmed a strong interaction between BPT1 and CBGA. A CBD yield of 6.92 mg/L was achieved, which was 100-fold higher than the yield generated by the starting strain. This study provides insights into high-level CBD-producing strain construction and lays the foundation for CBD supply.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.2c05468 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Estimating Model-Based Marginal Societal Health Benefits of Air Pollution Emission Reductions in the United States and Canada.
Res Rep Health Eff Inst
August 2024
Georgia Institute of Technology, Atlanta, Georgia, USA.
We developed spatially detailed source-impact estimates of population health burden measures of air pollution for the United States and Canada by quantifying sources-receptor relationships using the benefit-per-ton (BPT) metric. We calculated BPTs as the valuations of premature mortality counts due to fine particulate matter (PM; particulate matter ≤2.5 μm in aerodynamic diameter) exposure resulting from emissions of one ton of a given pollutant.
View Article and Find Full Text PDFBoosting the Cannabidiol Production in Engineered by Harnessing the Vacuolar Transporter BPT1.
J Agric Food Chem
September 2022
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing 100700, P. R. China.
Cannabidiol (CBD), the main nonpsychoactive cannabinoid in , has diverse applications in the pharmacological, food, and cosmetic industries. The long plantation period and the complex chemical structure of cannabidiol pose a great challenge on CBD supply. Here, we achieved biosynthesis of cannabidiol in .
View Article and Find Full Text PDFMolecular study on the role of vacuolar transporters in glycyrrhetinic acid production in engineered Saccharomyces cerevisiae.
Biochim Biophys Acta Biomembr
June 2022
Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China; Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China. Electronic address:
Glycyrrhetinic acid (GA) is one of the major bioactive components of the leguminous plant, Glycyrrhiza spp. (Chinese licorice). Owing to GA's complicated chemical structure, its production by chemical synthesis is challenging and requires other efficient strategies such as microbial synthesis.
View Article and Find Full Text PDFChaperonin point mutation enhances cadmium endurance in Saccharomyces cerevisiae.
Biotechnol Lett
September 2021
Molecular Genetics Laboratory, School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India.
Objective: To study the effect of the mutation in conserved G412E in Cct7p subunit of CCT complex on its cellular fate.
Results: TriC/CCT is a dynamic multimeric protein that assists in protein folding in an energy-dependent manner. A point mutation in the ATP binding pocket in the equatorial domain of the Cct7p subunit delays the doubling time.
Identification new potential multidrug resistance proteins of Saccharomyces cerevisiae.
J Microbiol Methods
September 2020
Department of Biotechnology, Polessky State University, Pinsk, Belarus.
ABC (ATP-binding cassette) proteins can transport metabolic molecules and removes metabolic products and xenobiotics from the cell. The important problem is to study activity and search inhibitors of ABC proteins. There is a problem that ABC-proteins can transport hydrophobic drugs across the cell membrane due to their high substrate specificity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!