Objective: Myricetin 3--galactoside is an active compound with pharmaceutical potential. The insufficient supply of this compound becomes a bottleneck in the druggability study of myricetin 3--galactoside. Thus, it is necessary to develop a biosynthetic process for myricetin 3--galactoside through metabolic engineering.
Methods: Two genes and encoding sucrose synthase and UDP-glucose 4-epimerase were introduced into BL21(DE3) to reconstruct a UDP--galactose (UDP-Gal) biosynthetic pathway in . The resultant chassis strain was able to produce UDP-Gal. Subsequently, a flavonol 3--galactosyltransferase gene was transformed into the chassis strain producing UDP-Gal. An artificial pathway for myricetin 3--galactoside biosynthesis was thus constructed in .
Results: The obtained engineered strain was demonstrated to be capable of producing myricetin 3--galactoside, reaching 29.7 mg/L.
Conclusion: Biosynthesis of myricetin 3--galactoside through engineered could be achieved. This result lays the foundation for the large-scale preparation of myricetin 3--galactoside.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476700 | PMC |
| http://dx.doi.org/10.1016/j.chmed.2020.03.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Key Metabolites Influencing Astringency and Bitterness in Yinghong 9 Large-Leaf Dark Tea Before and After Pile-Fermentation.
J Agric Food Chem
December 2024
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Understanding the impacts of pile-fermentation on the taste quality of dark tea (DT) is crucial. Although the large-leaf DT, Yinghong 9 DT, was successfully developed, its taste quality was not systematically studied. This research aims to analyze how pile-fermentation affects taste.
View Article and Find Full Text PDFNeuroprotective effects of walnut in nervous system disorders: A comprehensive review.
Iran J Basic Med Sci
January 2024
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Juglans regia L. (walnut) has a rich history in traditional medicine due to its various medicinal properties, including its neuroprotective effects on nervous system disorders. This updated review sheds light on the therapeutic potential of walnuts in nervous system disorders such as Alzheimer's disease, Parkinson's disease, depression, epilepsy, and pain, supported by evidence from in vivo and in vitro studies.
View Article and Find Full Text PDFStructured Fruit Cube Snack of BRS Vitoria Grape with Gala Apple: Phenolic Composition and Sensory Attributes.
Molecules
November 2024
Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha (UCLM), Avenue Camilo José Cela s/n, 13071 Ciudad Real, Spain.
This study developed a structured fruit cube (FC) snack using only natural ingredients, specifically red grape and apple, without hydrocolloids and sucrose. After development, physicochemical characterization and analysis of phenolic compounds (PCs), including anthocyanins, flavonols, and hydroxycinnamic acid derivatives, using HPLC-DAD-ESI-MS/MS, were conducted. Sensory quality was also assessed through an acceptance and sensory profile analysis using the rate-all-that-apply methodology.
View Article and Find Full Text PDFGreen Nanoengineered Fabrics: Waste-Derived Polyphenol-Zinc@ Silica Core-Shell Reactive Janus Nanoparticles for Functional Fabrics.
ACS Appl Mater Interfaces
July 2024
Leather Processing Technology Department, Council of Scientific and Industrial Research, Central Leather Research Institute (CSIR-CLRI), Adyar, Chennai 600020, India.
Fabricating Janus nanoparticle-functionalized fabrics with UV protection, strength enhancement, self-cleaning properties, and wash durability, with a biocompatible nature, is crucial in modern functional fabrics engineering. Particularly, tailoring multifunctional nanoparticles capable of exhibiting several distinct properties, utilizing low-cost raw materials, and adhering to green chemistry principles is pivotal. A fabrication strategy for developing multifunctional reactive Janus nanoparticles, utilizing waste-derived natural polyphenol (quercetin-3-glucuronide, myricetin-3-galactoside, gossypin, phlorizin, kaempferol, myricetin-3-arabinoside)-integrated zinc-silica core-shell Janus nanoparticles with UV protection, strength enhancement, and self-cleaning properties, is proposed.
View Article and Find Full Text PDFMetabolomics revealed metabolite biomarkers of antioxidant properties and flavonoid metabolite accumulation in purple rice after grain filling.
Food Chem X
June 2023
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China.
The correlation between flavonoids, phenolic metabolites and the total antioxidant capacity is well established. However, specific biomarkers of metabolites with antioxidant properties in purple rice grains remain unidentified. This study integrated nontargeted metabolomics, quantitative detection of flavonoids and phenolic compounds, and physiological and biochemical data to identify metabolite biomarkers of the antioxidant properties of purple rice grains after filling.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!