Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Objective: Plant hormones act as chemical messengers in the regulation of plant development and metabolism. The production of ginsenosides in hybrid is promoted by auxins that are transported and accumulated by PIN-FORMED (PIN) and PIN-LIKES (PILS) auxin transporters. However, genome-wide studies of PIN/PILS of ginseng are still scarce. In current study, identification and transcriptional profiling of / gene families, as well as their potential relationship with ginsenoside biosynthesis in were investigated.
Methods: / genes in was identified in silico genome-wide analysis, followed by phylogenetic relationships, gene structure, and protein profiles investigation. Moreover, previously reported RNA-sequence data from various tissues and roots after infection were utilized for / genes expression pattern analysis. The Pearson's correlation analysis of specific / genes expression level and main ginsenoside contents were taken to reveal the potential relationship between auxin transports and ginsenoside biosynthesis in .
Results: A genome-wide search of genome for homologous auxin transporter genes identified a total of 17 and 11 genes. Sequence alignment, putative motif organization, and sub-cellular localization indicated redundant and complementary biological functions of these / genes. Most / genes were differentially expressed in a tissue-specific manner, and showed significant correlations with ginsenoside content correspondingly. Eight auxin transporter genes, including both and subfamily members, were positively correlated with ginsenoside content (cor > 0.60; value <0.05). The expression levels of eleven auxin transporter genes were increased dramatically in the early stage (0-0.5 DPI) after infection, accompanied with various overall expression patterns, implying the dynamic auxin transport in response to biotic stress.
Conclusion: Based on the results, we speculate that the accumulation or depletion in temporal or spatial manner of auxin by PIN/PILS transporters involved in the regulation of HMGR activity and subsequent ginsenoside biosynthesis.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476816 | PMC |
http://dx.doi.org/10.1016/j.chmed.2021.08.001 | DOI Listing |
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