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In Vivo Detection of Cyclic-di-AMP in . | LitMetric
Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in (). In this study, we developed a novel biosensor ( P6-4) for in vivo detection of CDA in . The fluorescent biosensor is based on a natural CDA riboswitch from connected at its P6 stem to the dye-binding aptamer Spinach. Our study showed that P6-4 could detect a wide concentration range of CDA in both laboratory and clinical strains, making it suitable for use in both basic and clinical research applications.
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