Molecular detection of Rickettsia colombianensi in ticks (Acari, Ixodidae) collected from herpetofauna in San Juan de Carare, Colombia.

Int J Parasitol Parasites Wildl

Facultad de Ciencias Básicas, Grupo de Investigación Evolución, Sistemática y Ecología Molecular (GIESEMOL), Universidad Del Magdalena, Santa Marta, Colombia.

Published: December 2022

Knowledge about ticks and Rickettsiae in Colombia is still limited and the areas of the country where studies have been conducted are scarce. In this study, ticks (Acari, Ixodidae) associated with reptiles and amphibians in San Juan de Carare, Santander department, were morphologically and molecularly identified and tested for the presence of Rickettsia. For the molecular characterization of ticks, CO1, 12S and 16S sequences were generated and compared with other sequences available in genbank. Our analyses confirmed that the collected ticks were , and we provide the first report of this species parasitizing the snake . Of the samples analyzed, 69% were positive for sp. using the gltA, ompA and sca1 genes. Rickettsia sequences generated in this study were also compared to sequences downloaded from genbank by Bayesian inference (BI) and maximum likelihood (ML) phylogenetic analyzes. The presence of a single Rickettsia species, Rickettsia colombianensi, was identified. This study expands the knowledge regarding the distribution of ticks and Rickettsiae in Colombia.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474317PMC
http://dx.doi.org/10.1016/j.ijppaw.2022.08.004DOI Listing

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