Objective: To investigate the mechanisms of andrographolide against non-alcoholic steatohepatitis (NASH) based on network pharmacology, so as to provide a reference for further study of andrographolide in the treatment of NASH and other metabolic diseases.
Methods: The methionine- and choline-deficient (MCD) diet-induced NASH mice were treated by administration of andrographolide, and serum transaminase and pathological changes were analyzed. The network pharmacology-based bioinformatic strategy was then used to search the potential targets, construct protein-protein interaction (PPI) network, analyze gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment, and conduct molecular docking to explore the molecular mechanisms.
Results: The predicted core targets TNF, MAPK8, IL6, IL1B and AKT1 were enriched in non-alcoholic fatty liver disease (NAFLD) signaling pathway and against NASH by regulation of fatty acids synthesis, anti-inflammation and anti-oxidation.
Conclusion: This work provides a scientific basis for further demonstration of the anti-NASH mechanisms of andrographolide.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476713 | PMC |
http://dx.doi.org/10.1016/j.chmed.2021.05.001 | DOI Listing |
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