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Genome sizes of four important medicinal species in by flow cytometry. | LitMetric
Objective : Dianjixueteng is a geoherb in Yunnan Province, the source plant of which is . However, the formation of this geoherb is not clear in genetic mechanism, in which genome size is the first step that should be known on the genomic level. In this study we aimed to estimate the genome sizes of source plants of and three related herbs , , and . by flow cytometry (FCM) and make a comparison.Methods : The genome sizes of , , and . , i.e., the source plants of Dianjixueteng and its relative medicinal materials, were estimated by FCM. The nuclei of were isolated using modified LB01 buffer, for the rest species, by the Galbraith's buffer.Results : The genome sizes of , , , and were 7.36, 7.12, 7.01, and 5.15 pg/1C, respectively. Genome size of had no significant variation with those of and ( = 0.296), which was significantly larger than that of . .Conclusion : Genome size can not distinguish from and , but could distinguish them from , which lays the foundation for future studies on genetic mechanism of the geoherb formation.
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