L. is a traditional Chinese medicine used for the treatment of several different disease states including bacillary dysentery and enteritis. But importantly, it also plays a role as an anti-tumor agent. That said, little is known about the role endophytes play regarding the clinically bioactive metabolites in . In order to explore the effects of endophytic fungi on the accumulation, quality, and correlation in the content of the medicinal compounds, the structural diversity of endophytic fungi in was analyzed. The relationship between endophytes and quinolizidine alkaloids (QAs), housed within the seeds of , which were interpreted based on established methods of high-throughput sequencing and high-performance liquid chromatography. A total of 1,034,418 effective sequence reads and 257 operational taxonomic units (OTUs) were obtained from 33 samples which were sourced from 11 different sampling sites and further classified into 9 phyla, 20 classes, 45 orders, 85 families, and 118 genera. Ascomycota was found to be the dominant phylum of endophytic fungi in , with a relative abundance ranging from 60.85 to 98.30%. , , , and an unidentified Ascomycota were the core-shared endophytes, accounting for 49.96, 27.12, 14.83, and 7.88%, respectively. Correlation analysis showed that the Simpson's diversity index of endophytic fungal community in was significantly positively correlated with the Oxymatrine (OMA) content in different areas, while the Chao and Shannoneven indexes were significantly negatively correlated with OMA. The endophytic fungi of were positively correlated with the content of OMA, Oxysophocarpine (OSC), and total QAs. This study has mastered the endophytic fungi resources of , explored potential functional endophytic fungi, and provided a scientific basis for using biological fertilization strategies to improve the quality of .
Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, China.
is an important genus in the Apiosporaceae family with a worldwide distribution. They exhibit different lifestyles including pathogenic, saprophytic, and endophytic. In this study, we aimed to explore the associated with bamboo and collected 14 apiospora-like taxa from the forests of Yunnan Province, China.
Flowering of Henon bamboo ( var. ) was observed in Japan in 2020s. We estimated that the observation of flowering was recorded for the first time in 120 y.
The need for food production rises with the era of expanding population. As a result, there is more indirect demand for chemical pesticides and fertilizers. Serious environmental concerns result from the continuous and careless usage of chemicals.
AbstractTrait evolution depends both on the direct fitness effects of specific traits and on indirect selection arising from genetically correlated traits. Although well established in plants and animals, the role of trait correlations in microbial evolution remains a major open question. Here, we tested whether genetic correlations in a suite of metabolic traits are conserved between two sister lineages of fungal endophytes and whether phenotypic divergence between lineages occurred in the direction of the multivariate trait combination containing the most genetic variance within lineages (i.
The pine wood nematode (PWN), , is one of the most serious invasive forest pests, responsible for pine wilt disease (PWD). Currently, there are no effective, environmentally friendly control methods available. RNA interference (RNAi) technology has been extensively utilized to screen functional genes in eukaryotes and to explore sustainable pest management approaches through genetic engineering.
