Ethambutol (EMB) is a first-line antituberculosis drug currently being used clinically to treat tuberculosis. Mutations in the embCAB operon are responsible for EMB resistance. However, the discrepancies between genotypic and phenotypic EMB resistance have attracted much attention. We induced EMB resistance in and used an integrated genome-methylome-transcriptome-proteome approach to study the microevolutionary mechanism of EMB resistance. We identified 509 aberrantly methylated genes (313 hypermethylated genes and 196 hypomethylated genes). Moreover, some hypermethylated and hypomethylated genes were identified using RNA-seq profiling. Correlation analysis revealed that the differential methylation of genes was negatively correlated with transcription levels in EMB-resistant strains. Additionally, two hypermethylated candidate genes ( and ) were screened by iTRAQ-based quantitative proteomics analysis, verified by qPCR, and corresponded with DNA methylation differences. This is the first report that identifies EMB resistance-related genes in laboratory-induced mono-EMB-resistant using multi-omics profiling. Understanding the epigenetic features associated with EMB resistance may provide new insights into the underlying molecular mechanisms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471152 | PMC |
| http://dx.doi.org/10.3389/fcimb.2022.959911 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
QTL mapping provides new insights into emamectin benzoate resistance in salmon lice, Lepeophtheirus salmonis.
BMC Genomics
December 2024
Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
Background: The salmon louse (Lepeophtheirus salmonis) is a parasite of wild and farmed salmonid fish, causing huge economic damage to the commercial farming of Atlantic salmon (Salmo salar) in the northern hemisphere. The avermectin emamectin benzoate (EMB) is widely used for salmon delousing. While resistance to EMB is widespread in Atlantic populations of L.
View Article and Find Full Text PDFPerformance of a broth microdilution assay for routine minimum inhibitory concentration determination of 14 anti-tuberculous drugs against the complex based on the EUCAST reference protocol.
Antimicrob Agents Chemother
December 2024
Public Health Agency of Sweden, Solna, Sweden.
This comparative study aimed at qualifying a broth microdilution (BMD) assay for phenotypic drug susceptibility testing (pDST) of complex (MTBC) strains for implementation in a routine DST workflow. The assay was developed based on the EUCAST (European Committee on Antimicrobial Susceptibility Testing) reference protocol for determination of the minimum inhibitory concentration (MIC) of 14 anti-tuberculous drugs (isoniazid [INH], rifampicin [RIF], ethambutol [EMB], amikacin [AMI], moxifloxacin [MFX], levofloxacin [LFX], bedaquiline [BDQ], clofazimine [CFZ], delamanid [DLM], pretomanid [PA], para-aminosalicylic acid [PAS], linezolid [LZD], ethionamide [ETH], and cycloserine [CS]). Forty MTBC strains with various drug resistance profiles were tested to determine the agreement between MIC results and genotypic drug susceptibility testing (gDST) results derived from whole-genome sequencing (WGS).
View Article and Find Full Text PDFDetection of bla, bla, bla, and bla genes of antibiotic resistance in diarrheagenic E. coli causing enteric infection in hypertensive patients at Laquintinie Hospital, Littoral Region of Cameroon.
J Infect Public Health
December 2024
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address:
Article Synopsis
- Pathogenic Escherichia coli is a leading cause of diarrhea globally, and this study investigates how virulence genes and antibiotic resistance differ in hypertensive versus non-hypertensive patients.* -
- Researchers analyzed 518 fecal samples from patients in Douala, discovering that certain strains like enteropathogenic E. coli (EPEC) were more common in hypertensive individuals, while enteroaggregative E. coli (EAEC) predominated in non-hypertensives.* -
- The study found high resistance rates of E. coli to antibiotics, especially amoxicillin and sulfamethoxazole-trimethoprim, with a significant prevalence of extended-spectrum β-lactamase-producing strains (
Machine learning-based prediction of antibiotic resistance in Mycobacterium tuberculosis clinical isolates from Uganda.
BMC Infect Dis
December 2024
Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda.
Background: Efforts toward tuberculosis management and control are challenged by the emergence of Mycobacterium tuberculosis (MTB) resistance to existing anti-TB drugs. This study aimed to explore the potential of machine learning algorithms in predicting drug resistance of four anti-TB drugs (rifampicin, isoniazid, streptomycin, and ethambutol) in MTB using whole-genome sequence and clinical data from Uganda. We also assessed the model's generalizability on another dataset from South Africa.
View Article and Find Full Text PDFAnalysis of the Results of Tuberculosis Drug Resistance Surveillance in Yuexiu District, Guangzhou City, 2013-2022.
Immun Inflamm Dis
November 2024
State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, China.
Article Synopsis
- The study investigates drug resistance trends in tuberculosis patients in Yuexiu District, Guangzhou City, over a nine-year period from 2013 to 2022 to inform better prevention and control strategies.
- Out of 5,191 active tuberculosis patients analyzed, 1,659 Mycobacterium tuberculosis strains were cultured, resulting in 438 cases of drug resistance.
- The overall drug resistance rate was 26.40%, with significant findings including 15.37% monoresistance, 7.29% polyresistance, and 3.74% multidrug resistance, with the highest resistance observed to streptomycin and isoniazid.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!