AI Article Synopsis
- CircFBXW7 is implicated in various cancers, with its specific role in nonsmall cell lung cancer (NSCLC) being investigated.
- The study found that circFBXW7 expression is lower in NSCLC cells compared to normal lung epithelial cells, and its overexpression reduced cell growth, movement, and invasion capabilities.
- Additionally, miR-492 was identified as a target of circFBXW7, and miR-492 mimics counteracted the inhibitory effects of circFBXW7 on NSCLC cells.
Article Abstract
Background: CircFBXW7 has been determined to be involved in various cancers; however, its role in nonsmall cell lung cancer (NSCLC) remains unclear. This study examined the function and potential mechanism of circFBXW7 in NSCLC.
Methods: The structure of circFBXW7 was verified via RT-PCR and Sanger sequencing. The expression of circFBXW7 in NSCLC was determined by qRT-PCR. The effect of circFBXW7 overexpression on the proliferation, migration, and invasion of NSCLC cells was examined by CCK-8 and Transwell assays. Furthermore, a circFBXW7-miRNA network was established to explore their interaction. Predicted miRNA was determined by qRT-PCR. Moreover, the miRNA mimics were synthesized, wherein its effect on proliferation, migration, and invasion of NSCLC cells overexpressed circFBXW7 was assessed.
Results: The circularity of circFBXW7 was verified. The expression of circFBXW7 was found to be downregulated in NSCLC cells compared with that in normal human lung epithelial BEAS-2B cells. Overexpression of circFBXW7 reduced cell proliferation, migration, and invasion. Furthermore, according to the circFBXW7-miRNA network prediction and qRT-PCR validation, miR-492 was identified to be the target of circFBXW7. The inhibitory effect of circFBXW7 overexpression on cell proliferation, migration, and invasion was reversed by miR-492 mimics.
Conclusion: CircFBXW7 is downregulated in NSCLC. CircFBXW7 inhibits NSCLC cells proliferation, migration, and invasion by regulating miR-492.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9477579 | PMC |
| http://dx.doi.org/10.1155/2022/8699359 | DOI Listing |
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