[Establishment of an animal model of infection and study on therapeutic methods II Establishment of a mouse model of sparganosis mansoni via oral administration of procercoids].

Objective: To establish an animal model of sparganosis mansoni through oral administration of infected with procercoids.

Methods: Domestic cats were infected with under laboratory conditions, and fresh cat stool samples were collected, washed in dechlorinated water, and filtered. eggs were collected and prepared into suspensions. Twenty C57BL/6j mice were randomly divided into the experimental group ( = 15) and the control group ( = 5). Wild were infected with coracidia to allow 3 to 5 procercoids in each . Then, each mouse in the experimental group was given 15 infected with procercoids by gavage, while mice in the control group were orally administered with the same volume of dechlorinated water. All mice were sacrificed after 5 months, and dissected, and suspicious worms were collected. The serum specific IgG antibody against was measured in mice using enzyme-linked immunosorbent assay (ELISA). Genomic DNA was isolated from suspicious worms, and the specific cytochrome oxidase I () gene was amplified using PCR assay.

Results: Among the 15 mice in the experimental group, six were positive for the serum specific IgG antibody against , and milky white worms were found and collected from the subcutaneous regions of 4 out of 6 mice. Only one worm was detected in each mouse, and the worm morphology was similar to . Capillary electrophoresis of the PCR amplification products of gene presented a specific band with 151 bp in size, and sequencing analysis revealed 100% homology with .

Conclusions: A mouse model of sparganosis mansoni is successfully created through oral administration of infected with procercoids.