Previous studies on stationary phase gradients have inspired a new phase of theoretical work and an expansion of the concept to include multiple retention mechanisms. The theoretical work presented here corroborates previous reports that a stationary phase gradient can produce selectivity in a separation as effectively as careful adjustments to a mobile phase gradient program, especially when such column is operated in mobile phase gradient elution mode. In reality, no column is singularly based on one type of interaction mechanism, and many columns are nowadays designed to solicit multiple solute to stationary phase interactions. These mixed-mode columns inspired us to give more careful consideration to the idea of dual stationary phase gradients. With the theory applied here, it is suggested that the modulation of two unique interaction mechanisms across a chromatographic column has the potential to open up previously unseen selectivities. With the increasing prevalence of mixed-mode columns, we believe there will be ample opportunity to explore these new concepts in experimental work.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2022.463492 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolomic Analysis and Computational Biology Reveal the Potential Metabolic Pathways for Improvement of Fucoxanthin and Eicosapentaenoic Acid (EPA) Biosynthesis in Phaeodactylum tricornutum.
Appl Biochem Biotechnol
January 2025
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina - UFSC, Florianopolis, Santa Catarina, Brazil.
This work aims to provide a basis for the enhancement of fucoxanthin (FCX) and eicosapentaenoic acid (EPA) biosynthesis in the microalga Phaeodactylum tricornutum using metabolomics and computational biology. To achieve this, both targeted (UHPLC and GC-FID) and untargeted (FTIR and NMR) analyses were conducted throughout various stages of cell cultivation. Targeted analyses revealed that EPA concentrations peaked at the end of the logarithmic growth phase, while fucoxanthin levels remained consistent from the onset of this phase through to the stationary phase.
View Article and Find Full Text PDFHow do red foxes (Vulpes vulpes) explore their environment? Characteristics of movement patterns in time and space.
Mov Ecol
January 2025
Wildlife Research Unit Baden-Württemberg, LAZBW, Atzenberger Weg 99, 88326, Aulendorf, Germany.
Background: Many animals must adapt their movements to different conditions encountered during different life phases, such as when exploring extraterritorial areas for dispersal, foraging or breeding. To better understand how animals move in different movement phases, we asked whether movement patterns differ between one way directed movements, such as during the transient phase of dispersal or two way exploratory-like movements such as during extraterritorial excursions or stationary movements.
Methods: We GPS collared red foxes in a rural area in southern Germany between 2020 and 2023.
Spatial and temporal activity patterns of Amblyomma americanum.
Parasit Vectors
January 2025
Department of Entomology, Washington State University, 100 Dairy Road, Pullman, WA, USA.
Background: Estimates of tick abundance and distribution are used to determine the risk of tick-host contact. Tick surveys provide estimates of distributions and relative abundance for species that remain stationary and wait for passing hosts (i.e.
View Article and Find Full Text PDFChimera states in a system of stationary and flying-through deterministic particles with an internal degree of freedom.
Chaos
January 2025
Department of Control Theory, Scientific and Educational Mathematical Center "Mathematics of Future Technologies", Nizhny Novgorod State University, Gagarin Av. 23, Nizhny Novgorod 603022, Russia.
We consider the effect of the emergence of chimera states in a system of coexisting stationary and flying-through in potential particles with an internal degree of freedom determined by the phase. All particles tend to an equilibrium state with a small number of potential wells, which leads to the emergence of a stationary chimera. An increase in the number of potential wells leads to the emergence of particles flying-through along the medium, the phases of which form a moving chimera.
View Article and Find Full Text PDFOsteoporosis (OP) is a prevalent metabolic bone disease globally. Currently, the development of Traditional Chinese Medicine (TCM) resources to unblock joints, strengthen bones, and enhance muscle function to regulate anti-osteogenic and anabolic metabolism and thus reshape intraosseous homeostasis was an effective way to alleviate OP. The F-E-D formula, comprising Fructus Psoraleae, Eucommia, and Drynariae Rhizoma, has shown efficacy in treating OP.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!