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RSC and GRFs confer promoter directionality by restricting divergent noncoding transcription. | LitMetric

AI Article Synopsis

  • The study investigates how gene promoter directionality is influenced by the balance between protein-coding and noncoding transcription, highlighting its variability.
  • The chromatin remodelling complex RSC and general regulatory factors (GRFs) are shown to control this directionality by reducing divergent transcription relative to coding transcription.
  • By targeting specific transcription initiation sites, the research suggests that stably associated DNA-binding factors can act as barriers to divergent transcription, thereby enhancing promoter directionality.

Article Abstract

The directionality of gene promoters-the ratio of protein-coding over divergent noncoding transcription-is highly variable. How promoter directionality is controlled remains poorly understood. Here, we show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) dictate promoter directionality by attenuating divergent transcription relative to protein-coding transcription. At gene promoters that are highly directional, depletion of RSC leads to a relative increase in divergent noncoding transcription and thus to a decrease in promoter directionality. We find that RSC has a modest effect on nucleosome positioning upstream in promoters at the sites of divergent transcription. These promoters are also enriched for the binding of GRFs such as Reb1 and Abf1. Ectopic targeting of divergent transcription initiation sites with GRFs or the dCas9 DNA-binding protein suppresses divergent transcription. Our data suggest that RSC and GRFs play a pervasive role in limiting divergent transcription relative to coding direction transcription. We propose that any DNA-binding factor, when stably associated with cryptic transcription start sites, forms a barrier which represses divergent transcription, thereby promoting promoter directionality.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481977PMC
http://dx.doi.org/10.26508/lsa.202201394DOI Listing

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