AI Article Synopsis
- Extracellular vesicles (EVs) play a crucial role in cell communication and affect organ development and tissue differentiation in multicellular organisms.
- The study introduces an in vivo model using the fruit fly Drosophila melanogaster to visualize and analyze EV secretion through tissue-specific labeling and RNA interference (RNAi).
- Key findings indicate that EV secretion is primarily regulated by Rab11 and Rab35, while a novel role for Rab14 and the kinesin Klp98A in EV biogenesis and secretion was also identified.
Article Abstract
Extracellular vesicle (EV) secretion enables cell-cell communication in multicellular organisms. During development, EV secretion and the specific loading of signalling factors in EVs contributes to organ development and tissue differentiation. Here, we present an in vivo model to study EV secretion using the fat body and the haemolymph of the fruit fly, Drosophila melanogaster. The system makes use of tissue-specific EV labelling and is amenable to genetic modification by RNAi. This allows the unique combination of microscopic visualisation of EVs in different organs and quantitative biochemical purification to study how EVs are generated within the cells and which factors regulate their secretion in vivo. Characterisation of the system revealed that secretion of EVs from the fat body is mainly regulated by Rab11 and Rab35, highlighting the importance of recycling Rab GTPase family members for EV secretion. We furthermore discovered a so far unknown function of Rab14 along with the kinesin Klp98A in EV biogenesis and secretion.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473323 | PMC |
| http://dx.doi.org/10.1002/jev2.12263 | DOI Listing |
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