AI Article Synopsis
- The study investigates how a water-soluble porphyrin, HTOEtPyP4, and its manganese derivative, MnTOEtPyP4, bind to calf thymus DNA using various scientific techniques including UV/Vis spectrophotometry and Circular Dichroism.
- Results indicate that at low concentrations, porphyrins engage with DNA through one binding mode, while at higher concentrations, they display two distinct binding modes.
- Thermal melting analysis reveals that these porphyrins help stabilize the double-helical structure of DNA, and evidence of different melting curves suggests varying interactions based on concentration.
Article Abstract
The binding of water-soluble -tetra-(4N-oxyethylpyridyl) porphyrin (HTOEtPyP4) and its manganese (III) derivative (MnTOEtPyP4) with calf thymus DNA have been quantitatively studied using UV/Vis spectrophotometry, Circular Dichroism (CD), thermal melting curves and viscometry. The results show, that porphyrins interact with DNA via one binding mode at low relative concentrations () and two binding modes at high values of . The binding constant () and stoichiometry () were determined from binding isotherms for both porphyrin-DNA complexes. The thermal melting analysis indicates that the double-helical structure of DNA molecules is stabilizing in presence of studied porphyrins. At certain concentrations of porphyrin, two-stage melting curves were observed, which indicates the existence of two different binding modes. Obtained results show that MnTOEtPyP4 associates with DNA duplex via outside binding mode.Communicated by Ramaswamy H. Sarma.
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| http://dx.doi.org/10.1080/07391102.2022.2120076 | DOI Listing |
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