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Effects of lipoteichoic and arachidonic acids on the immune-regulatory mechanism of bovine mammary epithelial cells using multi-omics analysis. | LitMetric

AI Article Synopsis

  • The study investigates how lipoteichoic acid (LTA) from a mastitis-causing pathogen influences immune responses in dairy cows by triggering inflammation and avoiding immune detection.
  • It establishes a cell model using MAC-T cells to analyze the effects of both LTA and arachidonic acid (AA) on cell functions, specifically focusing on their concentrations and treatment durations.
  • Findings indicate that LTA regulates genes affecting metabolism, immune responses, and apoptosis, while AA appears to inhibit apoptosis and promote immune regulation, providing insights for future research on dairy cow mastitis.

Article Abstract

is one of the most important pathogens causing mastitis in dairy cows. It mainly utilizes the properties of its pathogenic factor, lipoteichoic acid (LTA), to elicit a host-cell inflammatory response and evade the host-cell immune response. Arachidonic acid (AA) has a regulatory role in the inflammatory response, cell metabolism, and apoptosis. The study aimed to establish a cell model by determining the optimal concentration of LTA and AA for cell induction using the Cell Counting Kit-8 assay and the quantitative polymerase chain reaction of ()β, , and . MAC-T cells were planted in 36 10-cm culture dishes at a density of 1 × 10 cells per dish. They were treated with LTA for 24 h to constitute the LTA group and with AA for 12 h to constitute the AA group. The cells were pretreated with LTA for 24 h followed by treatment with AA for 12 h to constitute the LTA + AA group. Using proteomic, transcriptomic, and metabolomic analyses, this study determined that LTA can regulate the expression of ()κ,and other genes to affect cellular metabolism, immune regulation and promote apoptosis. In contrast, AA was observed to regulate the expression of genes such as , and to inhibit cellular apoptosis and promote immune regulation, which provides a theoretical basis for future studies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450935PMC
http://dx.doi.org/10.3389/fvets.2022.984607DOI Listing

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