Anthracnose disease caused by is a serious disease that can afflict . Biological control is a rapidly growing approach for the management of plant diseases. In this study, we investigated the bio-control efficiency and the defense responses of an endophytic strain YYC 155, which was isolated from the root nodules of the against anthracnose disease, caused by in YYC 155 exhibited significant inhibitory activity against anthracnose disease, caused by in YYC 155 can secrete extracellular hydrolases, such as chitinase and β-1, 3-glucanase, which produce lipopeptides that are antimicrobial and forms strong biofilms. In addition, in treatment with YYC 155, the cell membranes of were injured and the leakage of cell contents from the mycelia of the pathogen was increased. Spraying 1 × 10 cells mL bacterial suspension of YYC 155 on leaves enhanced the activity of key enzymes in associated with the phenylpropanoid pathway and increased the content of phenolic compounds and flavonoids. Results of our study indicate that YYC 155 may potentially represent an effective biocontrol agent against anthracnose disease in .
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http://dx.doi.org/10.3389/fmicb.2022.956642 | DOI Listing |
Front Microbiol
August 2022
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.
Anthracnose disease caused by is a serious disease that can afflict . Biological control is a rapidly growing approach for the management of plant diseases. In this study, we investigated the bio-control efficiency and the defense responses of an endophytic strain YYC 155, which was isolated from the root nodules of the against anthracnose disease, caused by in YYC 155 exhibited significant inhibitory activity against anthracnose disease, caused by in YYC 155 can secrete extracellular hydrolases, such as chitinase and β-1, 3-glucanase, which produce lipopeptides that are antimicrobial and forms strong biofilms.
View Article and Find Full Text PDFSci Rep
August 2018
Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
Local acidification of stroma is proposed to favour pre-metastatic niche formation but the mechanism of initiation is unclear. We investigated whether Human Melanoma-derived exosomes (HMEX) could reprogram human adult dermal fibroblasts (HADF) and cause extracellular acidification. HMEX were isolated from supernatants of six melanoma cell lines (3 BRAF V600E mutant cell lines and 3 BRAF wild-type cell lines) using ultracentrifugation or Size Exclusion Chromatography (SEC).
View Article and Find Full Text PDFEndocrinology
March 2014
Departments of Cellular and Physiological Sciences (U.H.N., R.K.B., T.J.K.), Biochemistry and Molecular Biology (S.C., Y.Y.C.T., S.D.C., P.R.C.), and Surgery (T.J.K.), Life Sciences Institute, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
The ability of leptin to improve metabolic abnormalities in models of leptin deficiency, lipodystrophy, and even type 1 diabetes is of significant interest. However, the mechanism by which leptin mediates these effects remains ill-defined. Leptin was recently reported to regulate insulin-like growth factor-binding protein-2 (IGFBP2), and adenoviral overexpression of pharmacological levels of IGFBP2 ameliorates diabetic symptoms in many models of diabetes.
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