Characterization of KIRCD8 Regulatory T Cells in Humans by scRNA- and TCR-seq.

Previous studies have demonstrated the regulatory functions of Ly49CD8 T cells toward self-reactive CD4 T cells in mice. Recently, we found KIRCD8 T cells are the equivalent of mouse Ly49CD8 T cells in humans. They are increased in patients with autoimmune or infectious diseases as a negative feedback mechanism to suppress the arising pathogenic cells and maintain peripheral tolerance. Here, we describe the methods on how we characterize the KIRCD8 T cells from different diseases using single-cell RNA and TCR sequencing.