AI Article Synopsis
- Understanding the immunological synapse between peptide, HLA-E molecules, and TCR is key to effectively stimulate HLA-E-restricted T-cell responses in humans.
- The text outlines three methods for generating MHC-E/peptide complexes to explore their binding at a molecular level.
- These techniques include binding assays and using peptide-loaded HLA-E tetramers to detect and study peptide-specific human T-cells that are restricted by HLA-E.
Article Abstract
Understanding the interactions involved during the immunological synapse between peptide, HLA-E molecules, and TCR is crucial to effectively target protective HLA-E-restricted T-cell responses in humans. Here we describe three techniques based on the generation of MHC-E/peptide complexes (MHC-E generically includes HLA-E-like molecules in human and nonhuman species, while HLA-E specifically refers to human molecules), which allow to investigate MHC-E/peptide binding at the molecular level through binding assays and by using peptide loaded HLA-E tetramers, to detect, isolate, and study peptide-specific HLA-E-restricted human T-cells.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508831 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-2712-9_2 | DOI Listing |
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Article Synopsis
- Understanding the immunological synapse between peptide, HLA-E molecules, and TCR is key to effectively stimulate HLA-E-restricted T-cell responses in humans.
- The text outlines three methods for generating MHC-E/peptide complexes to explore their binding at a molecular level.
- These techniques include binding assays and using peptide-loaded HLA-E tetramers to detect and study peptide-specific human T-cells that are restricted by HLA-E.
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