AI Article Synopsis
- The article outlines methods for infecting adult and newborn mice with murine cytomegalovirus (MCMV) to study congenital CMV infection.
- It includes procedures for growing MCMV in cell cultures and preparing a more virulent strain from the salivary glands of infected mice.
- The article also describes a plaque assay technique to measure MCMV levels in infected tissues or virus stocks, along with methods for preparing murine embryonic fibroblasts for MCMV propagation.
Article Abstract
This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.
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