Poly-(lactic--glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Section .

Poly-(lactic--glycolic) acid (PLGA) is a biodegradable, biosafe, and biocompatible copolymer. The section causes otomycosis localized in the external auditory canal. In this research, , a species belonging to the section, was tested. Coumarin 6 and pterostilbene loaded in poly-(lactic--glycolic) acid nanoparticles (PLGA-coumarin6-NPs and PLGA-PTB-NPs) were tested for fungal cell uptake and antifungal ability against biofilm, respectively. Moreover, the activity of PLGA-PTB-NPs in inhibiting the infection was tested using larvae. The results showed a fluorescence signal, after 50 nm PLGA-coumarin6-NPs treatment, inside hyphae and along the entire thickness of the biofilm matrix, which was indicative of an efficient NP uptake. Regarding antifungal activity, a reduction in biofilm formation and mature biofilm with PLGA-PTB-NPs has been demonstrated. Moreover, in vivo experiments showed a significant reduction in mortality of infected larvae after injection of PLGA-PTB-NPs compared to free PTB at the same concentration. In conclusion, the PLGA-NPs system can increase the bioavailability of PTB in section biofilm by overcoming the biofilm matrix barrier and delivering PTB to fungal cells.