AI Article Synopsis

  • Cnidarians, like sea anemones and jellyfish, are known for their impressive ability to regenerate tissue, but the role of non-coding RNAs such as microRNAs in this process is not well understood.
  • Researchers sequenced the genome of a sea anemone from Hong Kong, achieving a substantial and improved assembly that sheds light on its genetic organization, particularly the arrangement of specific homeobox genes.
  • The study explored gene activity during the regeneration of tentacles, identifying potential contributors to this process, including Wnt signaling pathways, neuropeptides, and sesquiterpenoid pathway genes, which could lead to further insights into cnidarian biology and their response to environmental changes.

Article Abstract

Cnidarians including sea anemones, corals, hydra, and jellyfishes are a group of animals well known for their regeneration capacity. However, how non-coding RNAs such as microRNAs (also known as miRNAs) contribute to cnidarian tissue regeneration is poorly understood. Here, we sequenced and assembled the genome of the sea anemone collected in Hong Kong waters. The assembled genome size of is 229.21 Mb with a scaffold N50 of 10.58 Mb and BUSCO completeness of 91.1%, representing a significantly improved genome assembly of this species. The organization of ANTP-class homeobox genes in this anthozoan further supported the previous findings in jellyfishes, where most of these genes are mainly located on three scaffolds. Tentacles of were excised, and both mRNA and miRNA were sequenced at 9 time points (0 h, 6 h, 12 h, 18 h, 1 day, 2, 3, 6, and 8 days) from regenerating tentacles. In addition to the Wnt signaling pathway and homeobox genes that are shown to be likely involved in tissue regeneration as in other cnidarians, we have shown that GLWamide neuropeptides, and for the first time sesquiterpenoid pathway genes could potentially be involved in the late phase of cnidarian tissue regeneration. The established sea anemone model will be useful for further investigation of biology and evolution in, and the effect of climate change on this important group of animals.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444052PMC
http://dx.doi.org/10.3389/fcell.2022.900321DOI Listing

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