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A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. | LitMetric

AI Article Synopsis

  • Potassium ions (K+) are vital electrolytes in biological systems and understanding their role can enhance our knowledge of various processes.
  • Researchers reported the crystal structure of a K+ biosensor, GINKO1, and developed an enhanced version called GINKO2 through structure-guided optimization.
  • GINKO2 has improved sensitivity and specificity, enabling effective in vivo detection and imaging of K+ dynamics in different organisms like bacteria, plants, and mice.

Article Abstract

Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481166PMC
http://dx.doi.org/10.1371/journal.pbio.3001772DOI Listing

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