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The absence of the queuosine tRNA modification leads to pleiotropic phenotypes revealing perturbations of metal and oxidative stress homeostasis in Escherichia coli K12. | LitMetric

AI Article Synopsis

  • Queuosine (Q) is a special change in a part of bacteria that helps them deal with certain stressors, but scientists don’t know much about what happens without it.
  • In a study, a Q-deficient E. coli showed better resistance to nickel and cobalt but became more sensitive to cadmium compared to regular bacteria.
  • The lack of Q also changed how the bacteria reacted to nickel and caused increased stress inside the cells, leading to problems with oxygen levels and making them more vulnerable to hydrogen peroxide and related stressors.

Article Abstract

Queuosine (Q) is a conserved hypermodification of the wobble base of tRNA containing GUN anticodons but the physiological consequences of Q deficiency are poorly understood in bacteria. This work combines transcriptomic, proteomic and physiological studies to characterize a Q-deficient Escherichia coli K12 MG1655 mutant. The absence of Q led to an increased resistance to nickel and cobalt, and to an increased sensitivity to cadmium, compared to the wild-type (WT) strain. Transcriptomic analysis of the WT and Q-deficient strains, grown in the presence and absence of nickel, revealed that the nickel transporter genes (nikABCDE) are downregulated in the Q- mutant, even when nickel is not added. This mutant is therefore primed to resist to high nickel levels. Downstream analysis of the transcriptomic data suggested that the absence of Q triggers an atypical oxidative stress response, confirmed by the detection of slightly elevated reactive oxygen species (ROS) levels in the mutant, increased sensitivity to hydrogen peroxide and paraquat, and a subtle growth phenotype in a strain prone to accumulation of ROS.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508795PMC
http://dx.doi.org/10.1093/mtomcs/mfac065DOI Listing

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