AI Article Synopsis
- MicroRNAs (miRNAs) are important for regulating biological processes and can help diagnose diseases like psoriasis early.
- The study introduces a sensitive detection method for miRNAs using strand displacement amplification, focusing on miRNA-21 detection.
- This new fluorescent biosensor shows a broad detection range and a low limit of detection, suggesting its potential use in clinical diagnosis and biological research.
Article Abstract
MicroRNAs (miRNAs) play a pivotal role in regulating a variety of biological processes and can be used as biomarkers for the early diagnosis of various diseases, such as psoriasis. Herein, we depict a simple and sensitive miRNA detection method based on dual signal recycles, which is developed on the basis of strand displacement amplification (SDA). The sensor is successfully applied to the detection of miRNA-21 with a wide linear range from 100 fM to 10 nM and a lower limit of detection (LOD) of 67 fM. Because of the simple operation yet improved detection capability, we thereby believe that the developed fluorescent biosensor can be potentially applied for early clinical diagnosis as well as biological researches.
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| http://dx.doi.org/10.1007/s12010-022-04114-0 | DOI Listing |
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