AI Article Synopsis
- Intelectins are immune lectins found in chordates, with XCGL-1 being the first identified, crucial for fertilization membrane development.
- Researchers studied XCGL-1's biochemical properties, revealing its four-lobed structure and the significance of cysteines for disulfide bond formation.
- XCGL-1 binds galactose-containing carbohydrates with high affinity and the findings could lead to new applications in glycobiology.
Article Abstract
Intelectins are putative innate immune lectins that are found throughout chordates. The first intelectin reported was cortical granule lectin-1 (XCGL-1 or XL-35). XCGL-1 is critical in fertilization membrane development in . Here, we explored the biochemical properties of XCGL-1. The cysteines responsible for forming intermolecular disulfide bonds were identified. XCGL-1 adopted a four-lobed structure as observed by electron microscopy. The full-length XCGL-1 and the carbohydrate recognition domain (CRD) bind galactose-containing carbohydrates at nanomolar to micromolar affinities. Molecular modeling suggested that galactoside ligands coordinated the binding site calcium ion and interacted with residues around the groove made available by the non-conserved substitution compared to human intelectin-1. Folding conditions for production of recombinant XCGL-1 CRD were also investigated. Our results not only provide new biochemical insights into the function of XCGL-1, but may also provide foundation for further applications of XCGL-1 as glycobiology tools.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434060 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2022.e10396 | DOI Listing |
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