(L.) R. Br. ex Schult. and (Burm. F.) Merrill shoot cultures were treated with different concentrations of yeast extract (YE; 25-200 mg/L) and salicylic acid (SA; 50-200 µM), and their effect on lupeol production was assessed. The maximum dry weight (DW) biomass was recorded when shoots were treated with SA (50 µM) and shoots with YE (200 mg/L). Highest lupeol yield (335.40 ± 0.04 µg/g DW) was obtained in shoots after treatment with 50 µM of SA for 3 weeks. Whereas in , maximum lupeol content (584.26 ± 8.14 µg/g DW) was recorded by giving treatment with 25 μM of SA for 6 weeks.
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http://dx.doi.org/10.1080/14786419.2022.2119388 | DOI Listing |
Pharmaceuticals (Basel)
December 2024
Department of Biotechnology, BIODIATECH-Proplanta Research Centre for Applied Biotechnology in Diagnosis and Molecular Therapy, 400478 Cluj-Napoca, Romania.
: Pentacyclic triterpenoids, as bioactive phytochemicals, have proven to exhibit significant bioactivity (antioxidant, anti-inflammatory, hypoglycemic, and anticancer) and low cytotoxicity. This study developed convenient methods for extracting and characterizing a birch bark extract enriched in pentacyclic triterpenoids (betulin, betulinic acid, and lupeol) and entrapped in two bioavailable nanoformulations. The performance of ATR-FTIR spectroscopy as a cost-effective and non-destructive method was evaluated comparatively with accurate HPLC-based methods.
View Article and Find Full Text PDFComput Biol Med
January 2025
Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, Bangladesh. Electronic address:
Diabetes mellitus (DM) can be treated with various medications. However, individuals in underdeveloped countries may face challenges in using these treatments due to side effects and high costs. Anti-diabetic medications can inhibit enzymes, such as α-amylase, which is responsible for breaking down carbohydrates.
View Article and Find Full Text PDFPharmaceuticals (Basel)
December 2024
Department of Biotechnology, BIODIATECH-Proplanta Research Centre for Applied Biotechnology in Diagnosis and Molecular Therapy, 400478 Cluj-Napoca, Romania.
: Pentacyclic triterpenoids are increasingly studied as anticancer agents with many advantages compared to synthetic chemotherapeutics. The aim of this study was to prepare liposomal and nanostructured lipid formulations including a standardized extract of silver birch () outer bark (TTs) and to evaluate their potential as anticancer agents in vitro, using Melanoma B16-F10 and Walker carcinoma cells. : Appropriate solvents were selected for efficient TTs extraction, and original recipes were used to obtain Pegylated liposomes and nanolipid complexes with entrapped TTs, comparative to pure standards (betulinic acid and doxorubicin) in similar conditions.
View Article and Find Full Text PDFCurr Res Microb Sci
November 2024
Microbiology and Plant Pathology Laboratory, Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, India.
Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. and both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases.
View Article and Find Full Text PDFNat Prod Res
December 2024
Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory, Mons, Belgium.
Propolis is a resinous material collected by different bee species from various plant exudates and used to seal holes in honeycombs, smoothen the internal walls, embalm intruders, improve health and prevent diseases. From its -hexane extract, eight compounds were isolated and characterised as: mangiferonic acid (); 1-hydroxymangiferonic acid (), new natural product; mangiferolic acid(); 27-hydroxymangiferolic acid (), reported here for the first time as propolis constituent; 27-hydroxymangiferonic acid (); -amyrin (); -amyrin () and lupeol (). The chemical structures of the isolated compounds were elucidated using spectroscopic methods, such as 1D and 2D-NMR, mass spectrometry and comparison with previous published reports.
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