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Introduction: This study aimed to investigate the role of wingless-type MMTV integration site family member 5a (Wnt5a)-receptor tyrosine kinase-like orphan receptor 2 (Ror2) signaling in root resorption.
Methods: The messenger RNA (mRNA) expression of Wnt5a, Ror2, and RANKL in periodontal ligament cells (PDLCs) under compression force (CF) with or without Ror2 small interfering RNA (siRNA) were measured by quantitative reverse transcription-polymerase chain reaction, and these proteins released into culture supernatants were measured using enzyme-linked immunosorbent assay. Then these PDLC-conditioned media under CF with or without Ror2 siRNA were used to culture osteoclast precursors to detect osteoclastogenesis effects via tartrate-resistant acid phosphatase staining. In in vivo studies, the odontoclast number and the root resorption volume under excessive CF with or without Ror2 siRNA were investigated by tartrate-resistant acid phosphatase immunohistochemical staining and microcomputed tomography. The protein levels for Wnt5a, Ror2, and receptor activator of nuclear factor-kappa B ligand (RANKL) in the periodontal ligament tissues were also detected using immunohistochemical staining. Finally, the odontoclast number, root resorption volume, and the mRNA and protein expressions were compared between immature and mature teeth.
Results: The mRNA production and protein release level of Wnt5a, Ror2, and RANKL increased after CF, whereas they were significantly downregulated with Ror2 siRNA. The osteoclast number increased treating with culture medium from PDLC applying CF, but the increase was inhibited after adding Ror2 siRNA. In the animal model, the odontoclast number and root resorption volume significantly increased in the CF group but decreased in the CF with the Ror2 siRNA group. The protein levels of Wnt5a, Ror2, and RANKL in periodontal ligament were upregulated under excessive CF, and the pathway was inhibited with Ror2 siRNA. In the immature tooth group, the odontoclast number, root resorption volume, and the mRNA and protein expressions of Wnt5a-Ror2 signaling were reduced.
Conclusions: Wnt5a-Ror2 signaling in PDLCs enhanced by excessive CF could promote RANKL release and induce precursor differentiation, partly leading to increased odontoclast activity and ultimate root resorption. The less resorption of the immature tooth may be due to odontoclastogenesis inhibition by decreased expression of Wnt5a-Ror2 signaling.
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http://dx.doi.org/10.1016/j.ajodo.2022.05.011 | DOI Listing |
BMC Oral Health
March 2025
Department of Orthodontics, Cranial-Facial Growth and Development Center, Peking University School and Hospital of Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, PR China.
Objective: To explore the horizontal impact of extensive incisor retraction on alveolar bone and to investigate the relationship of changes in alveolar bone thickness (ΔBT) with horizontal retraction distance of the incisal edge (HRD) and horizontal retraction distance of alveolar ridge crest (HRD') in orthodontic treatment.
Materials And Methods: This retrospective study included 84 patients who underwent orthodontic extraction treatment, involving extensive incisor retraction (≥ 3 mm). Pre-treatment (T0) and post-treatment (T1) cone-beam computed tomography (CBCT) data were collected.
Arch Oral Biol
March 2025
São Paulo State University (UNESP), School of Dentistry, Araraquara - Laboratory of Histology and Embryology - Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Araraquara, SP, Brazil. Electronic address:
Objectives: To evaluate the histopathological features of induced periapical lesions (PL) exposed to the oral environment at different time points in developing molars of rats.
Methods: Twenty-four 30-day-old Holtzman rats had the pulp chamber of the mandibular left first molar opened and root canals were exposed to the oral environment. According to the exposure time, the animals were distributed into three groups (n = 8 rats/group): PL induced for 3 days (PLG-3d), PL induced for 1week (PLG-1w) and PL induced for 9 weeks (PLG-9w).
J Dent Res
March 2025
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Orthodontic root resorption (ORR) is a common yet significant complication of orthodontic treatment, largely driven by interactions between periodontal ligament cells (PDLCs) and M1 macrophages. Despite the clinical relevance of ORR, the role of mechanosensitive ion channels in PDLC-mediated ORR and the underlying mechanisms regulating inflammatory cell recruitment remain poorly understood. Here, we identified PIEZO1 as a critical mechanosensitive ion channel that modulates monocyte recruitment and ORR.
View Article and Find Full Text PDFSwiss Dent J
March 2025
Riga Stradiņš University, Faculty of Dentistry.
Autotransplantation of immature third molars is an effective treatment option, offering high success rates, minimal complications, and notable improvements in occlusal function and dental aesthetics. This study aims to review and evaluate the available evidence specifically on the survival and success rates of immature autotransplanted third molars and to identify prognostic factors that influence these outcomes. A comprehensive search was conducted in the Elsevier Journals (ScienceDirect), MEDLINE (PubMed), and Dentistry & Oral Sciences Source (EBSCOhost) databases up to May 2024.
View Article and Find Full Text PDFIndian J Otolaryngol Head Neck Surg
February 2025
Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh India.
Osteosarcoma, although the most common malignant bone tumor in long bones, represents only 5-8% of head and neck tumors, making it uncommon for clinicians. Conventional biopsy techniques can complicate diagnosis due to varying tissue representation in surrounding areas. This article discusses a case involving a 19-year-old female who presented with facial swelling and a rapidly growing intraoral mass.
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