The carcinogenic Rhodamine-B dye is recalcitrant which could cause serious hazards to human beings. Degradation with the application of unique bacterial strain is a sustainable technique. The bioremediation technique showed great potential to degrade a variety of recalcitrant pollutants like dyes. In this study, Brevundimonas diminuta, was selected for the breakdown of toxic textile dye Rhodamine-B. This bacterium showed 90-95% of degradation at the optimum conditions like 10 mg L of concentration of dye, pH 7 and temperature of 30 °C. Further UV-Visible spectrophotometry, FT-IR spectral scan, GC-MS analysis depicted the breakdown products like Methyl 18-fluoro-octadec-9-enoate, Methyl 18-fluoro-octadec-9-enoate and d-Homo-24-nor-17-oxachola-20,22-diene-3,16-dione,7-(acetyloxy)-1, 23 tri-epoxy-4,4,8-trimethyl. The degradation was confirmed by the changes in the functional groups, change in molecular weight and charge to-mass ratio. These results suggested that this strain is a deserving organism for the degradation of dye compounds.
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