Background: The present study determined the effect of the fractions obtained from scorpion venom on the tachyzoite of .
Methods: The fractions of dried venom of scorpion of Khuzestan Province, southern Iran in 2019 were isolated through gel filtration chromatography, and then tachyzoites were exposed to fractions of venom at different concentrations. Trypan blue counting and MTT were applied to assay tachyzoite viability, and the inhibition of the cellular growth of fractions in Vero cells was evaluated.
Results: The maximum effect on tachyzoite was observed in fraction 5 of venom. To further separate the protein, fraction 5 was used in high-performance liquid chromatography assay to purify its proteins. Based on the results of HPLC of fraction 5, among which the second peak, a peptide with <10 KDa representing a more potent effect in eliminating the tachyzoite of .
Conclusion: The scorpion venom-purified fractions possess anti-parasitic activity against the tachyzoite of and can be used in parasite-controlling studies.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375715 | PMC |
| http://dx.doi.org/10.18502/ijpa.v17i1.9019 | DOI Listing |
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