Brevinins exhibit a wide range of structural features and strong biological activities. Brevinin-2, derived from several amphibians, has shown antimicrobial activities. However, little is known about the wound-healing activity of brevinin-2. In this study, brevinin-2 cDNA was identified from the skin transcriptome of the dark-spotted frog (Pelophylax nigromaculatus) and it comprises a signal peptide, a propeptide, and a mature peptide. Sequence alignment with brevinin-2 derived from other amphibians showed variability of the mature peptide, and the presence of a C-terminal cyclic heptapeptide domain (Cys-Lys-Xaa4-Cys) in the mature peptide. Dark-spotted frog brevinin-2 belonged to the brevinin-2 cluster and was closely related to brevinin-2HB1 from Pelophylax hubeiensis. Synthetic dark-spotted frog brevinin-2 mature peptide (brevinin-2PN) exhibited antibacterial activity against several pathogens by destroying cell membrane integrity and hydrolysis of genomic DNA. Brevinin-2PN exhibited significant wound-healing activity by accelerating the healing of human skin fibroblast cell scratches, influencing cell migration, and stimulating gene expression of growth factors.
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Overexposure to ultraviolet light (UV) has become a major dermatological problem since the intensity of ultraviolet radiation is increasing. As an adaption to outside environments, amphibians gained an excellent peptide-based defense system in their naked skin from secular evolution. Here, we first determined the adaptation and resistance of the dark-spotted frogs ) to constant ultraviolet B (UVB) exposure.
View Article and Find Full Text PDFAn esculentin-1 homolog from a dark-spotted frog (Pelophylax nigromaculatus) possesses antibacterial and immunoregulatory properties.
BMC Vet Res
April 2024
College of Ecology, Lishui University, Lishui, 323000, China.
Background: Esculentin-1, initially discovered in the skin secretions of pool frogs (Pelophylax lessonae), has demonstrated broad-spectrum antimicrobial activity; however, its immunomodulatory properties have received little attention.
Results: In the present study, esculentin-1 cDNA was identified by analysing the skin transcriptome of the dark-spotted frog (Pelophylax nigromaculatus). Esculentin-1 from this species (esculentin-1PN) encompasses a signal peptide, an acidic spacer peptide, and a mature peptide.
Brevinin-2PN, an antimicrobial peptide identified from dark-spotted frog (Pelophylax nigromaculatus), exhibits wound-healing activity.
Dev Comp Immunol
December 2022
College of Ecology, Lishui University, Lishui, 323000, China. Electronic address:
Brevinins exhibit a wide range of structural features and strong biological activities. Brevinin-2, derived from several amphibians, has shown antimicrobial activities. However, little is known about the wound-healing activity of brevinin-2.
View Article and Find Full Text PDFBioevaluation and Targeted Modification of Temporin-FL From the Skin Secretion of Dark-Spotted Frog ().
Front Mol Biosci
October 2021
Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast, United Kingdom.
Bioactive proteins secreted by the granular glands of amphibian skin play a self-defensive role, and exhibit various bioactivities and . In light of the severity of the problem of antibiotic resistance for treating infections, many antimicrobial peptides (AMPs) have been developed and applied in clinical microbial treatments. We identified a naturally derived and potent antimicrobial peptide, temporin-FL, obtained from the skin secretion of via "shotgun" cloning.
View Article and Find Full Text PDFA revision of Callulops doriae (Anura: Microhylidae), with descriptions of four new species.
Zootaxa
May 2019
Department of Ecology and Evolutionary Biology, University of Michigan Ann Arbor, MI 48109, U.S.A.
I investigate the taxonomy of frogs currently assigned to the single species Callulops doriae, which is unique in the genus for its warty, dark-spotted dorsum and large yellow or orange blotches in the groin and on the hidden surfaces of the thighs. I show that many species are currently encompassed under this single name, I remove Manthophryne neuhaussi from synonymy, and I describe four new species, three of which have long been in museum collections, and one of which is newly collected by me. Each of these new species is most readily distinguished by their unique color patterns, but additional morphological features-primarily morphometric-also serve to distinguish among them.
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