AI Article Synopsis
- Spray-induced gene silencing (SIGS) uses small RNA molecules to target and silence essential genes in fungal pathogens, thereby controlling plant diseases.
- The stability of RNA in the environment poses challenges, but using BioClay, a formulation with clay particles, can enhance the durability and effectiveness of the delivered RNA.
- Research shows that BioClay significantly extends protection against the fungal pathogen Botrytis cinerea on tomato and chickpea plants compared to standard RNA treatments, marking SIGS as a promising eco-friendly alternative to chemical fungicides.
Article Abstract
One of the most promising tools for the control of fungal plant diseases is spray-induced gene silencing (SIGS). In SIGS, small interfering RNA (siRNA) or double-stranded RNA (dsRNA) targeting essential or virulence-related pathogen genes are exogenously applied to plants and postharvest products to trigger RNA interference (RNAi) of the targeted genes, inhibiting fungal growth and disease. However, SIGS is limited by the unstable nature of RNA under environmental conditions. The use of layered double hydroxide or clay particles as carriers to deliver biologically active dsRNA, a formulation termed BioClay™, can enhance RNA durability on plants, prolonging its activity against pathogens. Here, we demonstrate that dsRNA delivered as BioClay can prolong protection against Botrytis cinerea, a major plant fungal pathogen, on tomato leaves and fruit and on mature chickpea plants. BioClay increased the protection window from 1 to 3 weeks on tomato leaves and from 5 to 10 days on tomato fruits, when compared with naked dsRNA. In flowering chickpea plants, BioClay provided prolonged protection for up to 4 weeks, covering the critical period of poding, whereas naked dsRNA provided limited protection. This research represents a major step forward for the adoption of SIGS as an eco-friendly alternative to traditional fungicides.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464624 | PMC |
| http://dx.doi.org/10.1111/jipb.13353 | DOI Listing |
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